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| Registros recuperados : 12 | |
| 1. |  | PÉRES, I. A. H. F. S.; OLIVEIRA, C. E. de; TOMAS, W. M.; PELLEGRIN, A. O. Association between brucellosis prevalence and taxes of pampas deer (Ozotoceros bezoarticus) found without fawns in southwestern Nhecolândia region, Pantanal wetland, Brazil. BRUCELLOSIS RESEARCH CONFERENCE, 64., 2011, Buenos Aires. [Resumos]. [Buenos Aires: Asociación Argentina de Microbiologia], 2011. p. 105.| Biblioteca(s): Embrapa Pantanal. |
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| 2. | | PÉRES, I. A. H. F. S.; OLIVEIRA, C. E. de; ROSINHA, G. M. S.; SOARES, C. O.; TOMAS, W. M.; PELLEGRIN, A. O. Association between brucellosis prevalence and taxes of pampas deer (Ozotoceros bezoarticus) found without fawns in southwestern Nhecolândia region, Pantanal wetland, Brazil. In: BRUCELLOSIS 2011- INTERNATIONAL RESEARCH CONFERENCE; BRUCELLOSIS RESEARCH CONFERENCE, 64., 2011, Buenos Aires. [Anales]. Buenos Aires: Asociación Argentina de Microbiologia, 2011. p. 106 P 103.| Biblioteca(s): Embrapa Gado de Corte. |
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| 3. | | OLIVEIRA, C. E. de; PELLEGRIN, A. O.; TOMAS, W. M.; SOARES, C. O.; ARAUJO, F. R.; FUNES-HUACCA, M. E.; ROSINHA, G. M. S. Evidência molecular de Brucella sp. em Ozotoceros bezoarticus (veado campeiro) do Pantanal Sul-Mato-Grossense. Pesquisa Veterinária Brasileira, v. 30, n. 6, p. 503-509, jun. 2010. Na publicação: Carina Elisei, Aiesca Pellegrin.| Biblioteca(s): Embrapa Gado de Corte. |
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| 4. | | OLIVEIRA, C. E. de; PELLEGRIN, A. O.; TOMAS, W. M.; SOARES, C. O.; ARAUJO, F. R.; FUNES-HUACCA, M. E.; ROSINHA, G. M. S. Evidência molecular de Brucella sp. em Ozotoceros bezoarticus (veado campeiro) do Pantanal Sul-Mato-Grossense. Pesquisa Veterinária Brasileira, v.30, n.6, p. 503-509, junho, 2010| Biblioteca(s): Embrapa Pantanal. |
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| 5. | | GALVÃO, C. E.; FRAGOSO, S. P.; OLIVEIRA, C. E. de; FORNER, O.; PEREIRA, R. R. B.; SOARES, C. O.; ROSINHA, G. M. S. Identification of new Corynebacterium pseudotuberculosis antigens by immunoscreening of gene expression library. BMC Microbiology, v. 17, n. 202, p. 1-8, sept., 2017.| Biblioteca(s): Embrapa Gado de Corte. |
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| 6. | | OLIVEIRA, A. L. B. de; MACEDO, G. C. de; ROSINHA, G. M. S.; MELGAREJO, J. L.; ALVES, A. G. L.; BARRETO, W. T. G.; SANTOS, F. M.; CAMPOS, J. B. V.; HERRERA, H. M.; OLIVEIRA, C. E. de. Detection of Brucella spp. in dogs at Pantanal wetlands. Brazilian Journal of Microbiology, v. 50, n. 1, p. 307-312, 2019.| Biblioteca(s): Embrapa Gado de Corte. |
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| 7. | | REZENDE, M. B. de; HERRERA, H. M.; CARVALHO, C. M. E.; ANJOS, E. A. C.; RAMS, C. A. N.; ARAUJO, F. R.; TORRES, J. M.; OLIVEIRA, C. E. de. Detection of Leishmania spp. in Bats from an Area of Brazil Endemic for Visceral Leishmaniasis. Transboundary and Emerging Diseases, p. 1-7, 2017. SHORT COMMUNICATION| Biblioteca(s): Embrapa Gado de Corte. |
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| 8. | | VIEIRA, A. S.; ROSINHA, G. M. S.; VASCONCELLOS, S. A.; MORAIS, Z. M. DE.; VIANA, R. C.; OLIVEIRA, C. E. DE.; SOARES, C. O.; ARAUJO, F. R.; MOURAO, G. de M.; BIANCHI, R. DE C.; OLIFIERS, N.; RADEMAKER, V.; ROCHA, F. L.; PELLEGRIN, A. O. Identificação de mamíferos silvestres do Pantanal sul-mato-grossense portadores de Leptospira spp. Ciência Animal Brasileira v. 14, n. 3, p. 373-380, 2013. 8p.| Biblioteca(s): Embrapa Gado de Corte. |
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| 9. | | VIEIRA, A. S.; ROSINHA, G. M. S.; VASCONCELLOS, S. A.; MORAIS, Z. M. DE.; VIANA, R. C.; OLIVEIRA, C. E. DE.; SOARES, C. O.; ARAUJO, F. R.; MOURAO, G. de M.; BIANCHI, R. DE C.; OLIFIERS, N.; RADEMAKER, V.; ROCHA, F. L.; PELLEGRIN, A. O. Identificação de mamíferos silvestres do Pantanal sul-mato-grossense portadores de Leptospira spp. Ciência Animal Brasileira, v. 14, n. 3, p. 373-380, 2013. 8p.| Biblioteca(s): Embrapa Pantanal. |
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| 10. | | SANTOS, F. M.; MACEDO, G. C. DE; BARRETO, W. T. G.; OLIVEIRA-SANTOS, L. G. R.; GARCIA, C. M.; MOURAO, G. de M.; PORFÍRIO, G. E. de O.; MARINO, E. D.; ANDRE, M. R.; PERLES, L.; OLIVEIRA, C. E. de; ANDRADE, G. B. de; JANSEN, A. M.; HERRERA, H. M. Outcomes of Trypanosoma cruzi and Trypanosoma evansi infections on health of Southern coati (Nasua nasua), crab-eating fox (Cerdocyon thous), and ocelot (Leopardus pardalis) in the Brazilian Pantanal. Plos One, v. 13, n. 8, p. 1-15, 2018.| Biblioteca(s): Embrapa Pantanal. |
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| 11. | | VIEIRA, A. S.; ROSINHA, G. M. S.; OLIVEIRA, C. E. de; VASCONCELLOS, S. A.; LIMA-BORGES, P. A.; TOMAS, W. M.; MOURAO, G. de M.; LACERDA, A. C. R; SOARES, C. O.; ARAUJO, F. R.; PIOVEZAN, U.; ZUCCO, C. A.; PELLEGRIN, A. O. Survey of Leptospira spp in pampas deer (Ozotoceros bezoarticus) in the Pantanal wetlands of the state of Mato Grosso do Sul, Brazil by serology and polymerase chain reaction. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 106, n.6, p. 763 - 768, Sept. 2011.| Biblioteca(s): Embrapa Gado de Corte. |
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| 12. | | VIEIRA, A. S.; ROSINHA, G. M. S.; OLIVEIRA, C. E. de; VASCONCELLOS, S. A.; LIMA-BORGES, P. A.; TOMAS, W. M.; MOURAO, G. de M.; LACERDA, A. C. R; SOARES, C. O.; ARAÚJO, F. R. de; PIOVEZAN, U.; ZUCCO, C. A.; PELLEGRIN, A. O. Survey of Leptospira spp in pampas deer (Ozotoceros bezoarticus) in the Pantanal wetlands of the state of Mato Grosso do Sul, Brazil by serology and polymerase chain reaction. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 106, n.6, p. 763 - 768, sep. 2011.| Biblioteca(s): Embrapa Pantanal. |
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| Registros recuperados : 12 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Gado de Corte. |
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Data corrente: |
10/11/2017 |
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Data da última atualização: |
13/11/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
GALVÃO, C. E.; FRAGOSO, S. P.; OLIVEIRA, C. E. de; FORNER, O.; PEREIRA, R. R. B.; SOARES, C. O.; ROSINHA, G. M. S. |
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Afiliação: |
CLEBER EDUARDO GALVÃO, Federal University of Mato Grosso do Sul (UFMS), Campo Grande, MS.; STENIO PERDIGÃO FRAGOSO, Carlos Chagas Institute, Oswaldo Cruz Foundation (ICC-FIOCRUZ), Curitiba, PR.; CARINA ELISEI DE OLIVEIRA, Dom Bosco Catholic University (UCDB), Campo Grande, MS.; ODINÉIA FORNER, Federal University of Paraná (UFPR), Curitiba, PR.; RENATA RIBEIRO BASTOS PEREIRA, Federal University of Mato Grosso do Sul (UFMS), Campo Grande, MS.; CLEBER OLIVEIRA SOARES, DE/TT; GRACIA MARIA SOARES ROSINHA, CNPGC. |
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Título: |
Identification of new Corynebacterium pseudotuberculosis antigens by immunoscreening of gene expression library. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
BMC Microbiology, v. 17, n. 202, p. 1-8, sept., 2017. |
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DOI: |
10.1186/s12866-017-1110-7 |
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Idioma: |
Inglês |
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Conteúdo: |
Background: Caseous lymphadenitis (CLA) is a disease that affects sheep, goats and occasionally humans. The etiologic agent is the Corynebacterium pseudotuberculosis bacillus. The objective of this study was to build a gene expression library from C. pseudotuberculosis and use immunoscreening to identify genes that encode potential antigenic proteins for the development of DNA and subunit vaccines against CLA. Results: A wild strain of C. pseudotuberculosis was used for extraction and partial digestion of genomic DNA. Sequences between 1000 and 5000 base pairs (bp) were excised from the gel, purified, and the digested DNA fragments were joined to bacteriophage vector ZAP Express, packaged into phage and transfected into Escherichia coli . For immunoscreening a positive sheep sera pool and a negative sera pool for CLA were used. Four clones were identified that strongly reacted to sera. The clones were confirmed by polymerase chain reaction (PCR) followed by sequencing for genomic comparison of C. pseudotuberculosis in GenBank. The genes identified were dak2, fagA, fagB, NlpC/P60 protein family and LPxTG putative protein family. Conclusion: Proteins of this type can be antigenic which could aid in the development of subunit or DNA vaccines against CLA as well as in the development of serological tests for diagnosis. Immunoscreening of the gene expression library was shown to be a sensitive and efficient technique to identify probable immunodominant genes. |
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Palavras-Chave: |
DNA vaccines. |
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Thesagro: |
Corynebacterium Pseudotuberculosis. |
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Thesaurus NAL: |
Caseous lymphadenitis; Gene expression; Sheep. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/166571/1/Identification-of-new-Corynebacterium.pdf
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Marc: |
LEADER 02301naa a2200265 a 4500
001 2079448
005 2017-11-13
008 2017 bl uuuu u00u1 u #d
024 7 $a10.1186/s12866-017-1110-7$2DOI
100 1 $aGALVÃO, C. E.
245 $aIdentification of new Corynebacterium pseudotuberculosis antigens by immunoscreening of gene expression library.$h[electronic resource]
260 $c2017
520 $aBackground: Caseous lymphadenitis (CLA) is a disease that affects sheep, goats and occasionally humans. The etiologic agent is the Corynebacterium pseudotuberculosis bacillus. The objective of this study was to build a gene expression library from C. pseudotuberculosis and use immunoscreening to identify genes that encode potential antigenic proteins for the development of DNA and subunit vaccines against CLA. Results: A wild strain of C. pseudotuberculosis was used for extraction and partial digestion of genomic DNA. Sequences between 1000 and 5000 base pairs (bp) were excised from the gel, purified, and the digested DNA fragments were joined to bacteriophage vector ZAP Express, packaged into phage and transfected into Escherichia coli . For immunoscreening a positive sheep sera pool and a negative sera pool for CLA were used. Four clones were identified that strongly reacted to sera. The clones were confirmed by polymerase chain reaction (PCR) followed by sequencing for genomic comparison of C. pseudotuberculosis in GenBank. The genes identified were dak2, fagA, fagB, NlpC/P60 protein family and LPxTG putative protein family. Conclusion: Proteins of this type can be antigenic which could aid in the development of subunit or DNA vaccines against CLA as well as in the development of serological tests for diagnosis. Immunoscreening of the gene expression library was shown to be a sensitive and efficient technique to identify probable immunodominant genes.
650 $aCaseous lymphadenitis
650 $aGene expression
650 $aSheep
650 $aCorynebacterium Pseudotuberculosis
653 $aDNA vaccines
700 1 $aFRAGOSO, S. P.
700 1 $aOLIVEIRA, C. E. de
700 1 $aFORNER, O.
700 1 $aPEREIRA, R. R. B.
700 1 $aSOARES, C. O.
700 1 $aROSINHA, G. M. S.
773 $tBMC Microbiology$gv. 17, n. 202, p. 1-8, sept., 2017.
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Embrapa Gado de Corte (CNPGC) |
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